By Evelyn M. Rusli 

Yik Yak, the controversial anonymous-messaging app that has spread rapidly across college campuses, is proof that it can take as little as a year these days to go from zero to a valuation of hundreds of millions of dollars.

Sequoia Capital has led a $62 million investment in Yik Yak in the Atlanta-based startup's third funding round this year, according to people familiar with the deal. Current investors are expected to participate in the round.

The funding round values Yik Yak at between $300 million and $400 million, according to people familiar with the matter.

The deal is a sign of investors' eagerness to plow money into social and messaging apps, despite how crowded the market has become. The $22 billion sale of WhatsApp to Facebook Inc. in February and Snapchat's rising valuation--which recently hit $10 billion--has further stoked investor interest this year.

For Sequoia, which reaped one of the largest payouts in venture-capital history this year with WhatsApp, the Yik Yak investment marks another bet on a hot messaging app. The WhatsApp deal likely earned Sequoia more than $3 billion from a $60 million investment, a person familiar with the deal has said.

Jim Goetz, the partner who led Sequoia's WhatsApp investment three years ago, is joining Yik Yak's board, said people familiar with the matter.

Yik Yak, started by two recent graduates of Furman University in South Carolina, isn't a household name. But the service has grown rapidly across campuses since its debut last November. At the same time it has drawn criticism from high schools, who say it is a breeding ground for cyberbullying.

The service acts as an anonymous local bulletin board. People located within the same area, such as a campus or a part of a city, can post messages and replies of as many as 200 characters to one another without names or aliases. The messages can range from the mundane to the scandalous--and everything in between.

While the app has focused on college students, some high-school students have used the service to bully and slander their peers, and in some cases, prank their schools. In response, the startup has tried to restrict the app's use through geo-fencing, building virtual walls around the addresses of high schools.

Though the effort has worked in many locations, the company has struggled to snuff out the problem. Last week, for instance, a high school in Southern California was closed for two days after a Yik Yak user posted a message threatening the school. The same week, three teens were arrested in Pleasant Hill, Iowa, after similar threats surfaced.

According to Yik Yak co-founder Tyler Droll, high-school students haven't shown the maturity to use his app. He said that until Yik Yak has better structures in place to handle potential problems, he will try to keep the app out of high schools. Yik Yak requires users to be 18 years or older, or 17 if permitted by a parent or guardian.

The app is yet another take on anonymous and ephemeral messaging, a trend that has erupted in the wake of Snapchat's success.

While there are significant differences between Yik Yak and Snapchat, a private chat service that allows users to send self-destructing messages, both are a reaction to social networks like Facebook, where a user's posts are tied to his real identity.

By contrast, both Yik Yak and Snapchat are aimed at making it easier for users to share content with less fear of judgment. Two other apps, Secret and Whisper, have emerged this year as new takes on anonymous messaging and raised tens of millions of dollars in capital.

The story of Yik Yak's climb reads like a startup cliché.

Yik Yak was founded by Mr. Droll, 24 years old, and Brooks Buffington, 23, who met as students at Furman. Like Facebook chief Mark Zuckerberg and Snapchat Chief Excecutive Evan Spiegel, Messrs. Droll and Buffington started the company as young 20-somethings.

Yik Yak is now on roughly 1,500 college campuses and consistently ranks in the top 60 free apps in the Apple Inc. app store.

After graduation, the co-founders decided to work on their app, eschewing more traditional careers in finance and health. And, like Facebook, the startup leveraged the close-knit nature of colleges to rapidly attract users and spread by word-of-mouth. After launching at Furman, Yik Yak spread to nearby Wofford College and then region to region, soon moving further south, then all along the East Coast, before hopping west.

Every time there was a break in classes, such as during a holiday, the app would spread further as users met up with friends from home, according to an interview with Messrs. Droll and Buffington last month. Before the summer break this year, there were about 200 college campuses on the app; when classes resumed in the fall, the app jumped to roughly a thousand campuses, Mr. Droll said.

Like Facebook and Snapchat before, Yik Yak has also wrestled with its own founder drama. This month, it was sued by a former classmate and fraternity brother of Messrs. Droll and Buffington who says he is also a co-founder of the company. Yik Yak has said it intends to fight the lawsuit vigorously.

As money continues to pour into Yik Yak, it remains to be seen if the app can grow beyond its core demographic and stamp out its use in bullying. The startup is trying to be relevant to postcollege users, such as those in densely populated cities. However, cities lack the same unifying elements of a college campus, because they are comprised of more diverse groups of people.

Yik Yak's previous backers include DCM, Azure Capital Partners, Vaizra Investments, Atlanta Ventures, Renren Inc. and venture capitalist Tim Draper. Before the latest financing round, the startup raised two investments this year totaling $11.5 million. In a sign of how fast investor interest has bloomed, the first investment in April valued the company at roughly $10 million, according to one person with knowledge of the matter.

Write to Evelyn M. Rusli at evelyn.rusli@wsj.com

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