DUBLIN, Sept. 18, 2017 /PRNewswire/
-- HUPO2017 -- At the 16th Annual World Congress
of the Human Proteome Organization (HUPO), Bruker
introduces the timsTOF Pro system for PASEF mass
spectrometry, using proprietary trapped ion mobility spectrometry
(TIMS) technology for higher-speed, higher-sensitivity and robust
shotgun proteomics with outstanding single-shot peptide and protein
identification performance.
The novel timsTOF Pro is particularly advantageous
also for quantitative proteomics workflows, as this innovative
TIMS-powered quadrupole time-of-flight mass spectrometer (QTOF-MS)
is supported by MaxQuant/Perseus and PEAKS Studio
proteome analysis software.
The unique front-end TIMS analyzer is optimized for higher-speed
shotgun proteomics from smaller sample amounts with outstanding
identification performance. Its unique dual TIMS geometry allows
ions to be accumulated in parallel in the first TIMS section, and
after an additional TIMS separation step in real time, the ions are
released from the second TIMS section for MS/MS fragmentation.
This results in nearly 100% duty cycle, giving this parallel
accumulation and serial fragmentation (PASEF) technique
unprecedented performance for reproducible nanoflow LC-MS analysis
of enzymatically digested protein mixtures.
The PASEF capability represents a step-change improvement in
performance as it delivers higher-sensitivity and higher-speed
shotgun proteomics without loss of mass resolution. Higher scan
speeds result in lower mass resolution in FT-based MS technology
commonly used for shotgun proteomics. These critical limitations
are eliminated by PASEF, allowing for a duty cycle near 100% with
high sensitivity while maintaining ultra-high mass resolution for
both the precursor and the product ions. This key PASEF capability
leveraging the dual TIMS technology gives scientists the tools to
dig deeper into the complex biology of the cellular machinery and
the potential to discover low-level, biologically significant
proteins, or validate them in translational and clinical proteomics
research on large cohort sizes and in longitudinal studies.
Quantitative proteomics is a key area of research in proteomics,
and dual TIMS-powered PASEF provides an inherent advantage over the
limitations of gated, tandem-in-time, FT-based MS analyzers. The
new timsTOF Pro delivers over four orders of
magnitude of dynamic range with low peptide loads (100-200 ng),
which makes it very suitable for proteomics on the small cell
populations and low sample amounts often encountered in biological
and clinical research.
Matthias Mann, Director of the
Department of Proteomics and Signal Transduction at the Max Planck
Institute of Biochemistry in Martinsried, Germany, stated: "My lab has collaborated with
Bruker in the development of the PASEF technology, and we are
delighted to see the timsTOF Pro realize the
potential for shotgun proteomics that we had originally envisioned.
This new technology has the potential to revolutionize several
areas in proteomics: clinical research where speed and robustness
are key for running large sample cohorts; applications requiring
increased sensitivity, for e.g. samples enriched in phosphopeptides
or where limited numbers of cells are available for analysis; and
for quantitation experiments using isobaric tags, where the
additional separation using trapped ion mobility can at least
partially remove interference from co-fragmenting species that
cause the so-called ratio compression effect that leads to
unacceptable errors. We are excited about the potential for further
developments of the technology as well."
Gary Kruppa, Vice President for
Omics Solutions at Bruker Daltonics, said: "Proteomics researchers
always want improvements in speed, sensitivity and quantitative
capabilities, while maintaining the advantages of high-resolution,
accurate mass and isotopic pattern fidelity so they can go deeper
into the proteome. With PASEF, scientists now don't have to trade
off resolution for scan speed and sensitivity – they get the triple
benefit of high-sensitivity, high-scan speed, and unrivaled
specificity through TIMS precursor ion selection. Together, this
provides the exciting possibility of discovering low level
biologically relevant proteins that currently are beyond the
performance envelope of non-TIMS mass spectrometers."
Juergen Cox, Group Leader of the
Computational Systems Biochemistry Group at the Max Planck
Institute of Biochemistry in Martinsried, commented: "Bruker's
decision to adopt an open-file format so that we can directly work
with raw data will benefit the community of proteomics researchers
using MaxQuant and Perseus. We are impressed with the
quality of the data, and look forward to working with Bruker to
support mutual customers with state-of –the-art software for the
analysis and statistical interrogation of data acquired on the
timsTOF Pro with PASEF."
About the timsTOF PRO with PASEF
The proprietary timsTOF Pro system uses
PASEF, enabled by Trapped Ion Mobility Spectrometry (TIMS) to
provide industry-leading data acquisition speed for shotgun
proteomics. The unique dual TIMS geometry of the timsTOF
Pro, combined with the time focusing of the ion packets in
the TIMS device, means that the speed advantage provided by PASEF
comes along with simultaneous improvements in sensitivity and
quantitation. All of these gains in speed, sensitivity and
quantitation maintain the advantages of Bruker's high-performance
QTOF mass spectrometers, including high mass resolution (resolving
power of 50,000 FWHM even at highest data acquisition rates), ppm
accurate mass, and high isotopic fidelity (True Isotopic Pattern,
or TIPTM). The robust timsTOF Pro with
PASEF gives scientists the tools to dig deeper into the complex
biology of the cellular machinery and the potential to discover
low-level, biologically significant proteins, or validate them in
translational and clinical proteomics research.
About the MaxQuant and Perseus software
platforms
MaxQuant is the industry standard in shotgun proteomics
data analysis. Developed over the last decade by Juergen Cox, it has become the most used package
for the identification and quantification of peptides, proteins and
posttranslational modifications. Recently, MaxQuant has been
adapted to the analysis of timsTOF data, managing 4D features in
the space spanned by retention time, ion mobility, mass and signal
intensity. The Perseus software for multi-omics data
analysis supports biological and biomedical researchers in
interpreting molecular quantification, interaction and protein
post-translational modification data. Perseus contains
a comprehensive portfolio of statistical tools for high-dimensional
data analysis covering normalization, pattern recognition,
time-series analysis, cross-omics comparisons and
multiple-hypothesis testing. For more information:
http://www.biochem.mpg.de/5111795/maxquant.
About PEAKS Studio
PEAKS Studio, the flagship software of Bioinformatics
Solutions, Inc., offers an innovative mass spectrometry data
analysis workflow to the proteomics research community. Since its
debut in the early 2000s, PEAKS Studio has been highly recognized
for its benchmarking de novo sequencing algorithm, which was
integrated in all other software modules for shotgun proteomics.
The combination of de novo sequencing with traditional
database searches ensures a complete interpretation of raw spectral
data to embrace the complexity and sensitivity of mass
spectrometry, and offers advanced solutions for proteomic and
therapeutic protein discovery as provided through peptide/protein
identification and quantification, peptide mapping,
post-translational modifications and sequence variants.
The unique workflow offered by PEAKS Studio together with
the compelling data quality derived by fourth-dimension ion
mobility of the timsTOF Pro has sparked
Bioinformatics Solutions, Inc. and Bruker to establish
collaborative work. For more information: www.bioinfor.com
About Bruker Corporation (NASDAQ:
BRKR)
For more than 55 years, Bruker has enabled scientists to make
breakthrough discoveries and develop new applications that improve
the quality of human life. Bruker's high-performance scientific
instruments and high-value analytical and diagnostic solutions
enable scientists to explore life and materials at molecular,
cellular and microscopic levels.
In close cooperation with our customers, Bruker is enabling
innovation, productivity and customer success in life science
molecular research, in applied and pharma applications, and in
microscopy, nano-analysis and industrial applications. In recent
years, Bruker has also become a provider of high-performance
systems for cell biology, preclinical imaging, clinical phenomics
and proteomics research, clinical microbiology, and for molecular
pathology research. For more information: www.bruker.com
Media
Contact:
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Investor
Contact:
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Petra
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Miroslava
Minkova
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Daltonics Division
Marketing & Communications
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Bruker Head of
Investor Relations
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T: +49 (421)
2205-2843
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T: +1 (978) 663-3660,
ext. 1479
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E:
petra.scheffer@bruker.com
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E:
miroslava.minkova@bruker.com
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